HSP70在牦牛肠道中的分布与变化规律

doi:10.11843/j.issn.0366-6964.2018.08.019

Abstract

Abstract:

In order to explore the distribution and change rule of HSP70 in yak intestine, six healthy adult yaks (3-6 years old) were selected, and the distribution and change rule of HSP70 in yak intestine were observed and detected by immunohistochemistry(IHC), Western blotting (WB) and real-time fluorescence quantitative PCR (RT-qPCR) methods. Immunohistochemical results showed that HSP70 was expressed in the mucosa, submucosa and muscular layer of yak small intestine, among which the columnar cells of small intestinal mucosa epithelium were strongly positive, but the goblet cells were negative. The intestinal glandular cells of the intestinal glands and the smooth muscle cells of the muscular layer also showed positive expression, and the positive particles were mainly distributed in the cytoplasm and nucleus of the above cells. Similar to the small intestine, HSP70 was also expressed in the mucosa, submucosa and muscular layer of yak large intestine. The positive reaction was mainly located in scattered connective tissue cells of the mucosal epithelium and lamina propria,while positive reaction of colonic gland cells in the lamina propria was found only in the nucleus, but not in the cytoplasm. The statistical analysis of Western blotting and integrated optical density value of immunohistochemistry showed that, from the anterior segment of small intestine to the distal segment of large intestine, the expression of HSP70 increased first and then decreased, and peaked in the cecum. In addition, the relative protein expression of HSP70 in the cecum and colon was significantly higher than that of other bowel segments (P<0.01). Real-time fluorescent quantitative PCR showed that HSP70 mRNA had the highest relative expression in the cecum, which was twice as much as that of the duodenum, 1.68 times of the jejunum, 1.06 times of the ileum, 1.43 times of the colon and 1.52 times of that of the rectum. This study revealed that HSP70 showed a broad positive reaction in yak intestine, and HSP70 gene, HSP70 protein had different expression patterns in the small intestine and the large intestine of yaks. The results revealed the similarities and differences in biological functions played by HSP70 protein between the small intestine and the large intestine and provided information for the study of the expression and function analysis of HSP70 in ruminant tissues.

CLC Number:

S852.16

LIAO Bo, CUI Yan, YANG Xue, HE Jun-feng, SONG Liang-li, YANG Xiao-qing, LIU Peng-gang. Distribution and Change Rule of HSP70 in Yak Intestine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1735-1742.

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Distribution and Change Rule of HSP70 in Yak Intestine

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